A Highly Sensitive Europium Nanoparticle-Based Immunoassay for Detection of Influenza A/B Virus Antigen in Clinical Specimens
Identifieur interne : 000A54 ( Main/Exploration ); précédent : 000A53; suivant : 000A55A Highly Sensitive Europium Nanoparticle-Based Immunoassay for Detection of Influenza A/B Virus Antigen in Clinical Specimens
Auteurs : Panhe Zhang [États-Unis] ; Sai Vikram Vemula [États-Unis] ; Jiangqin Zhao [États-Unis] ; Bingchen Du [États-Unis] ; Haleyurgirisetty Mohan [États-Unis] ; Jikun Liu [États-Unis] ; Haja Sittana El Mubarak [États-Unis] ; Marie L. Landry [États-Unis] ; Indira Hewlett [États-Unis]Source :
- Journal of Clinical Microbiology [ 0095-1137 ] ; 2014.
Abstract
We report the development of a novel europium nanoparticle-based immunoassay (ENIA) for rapid detection of influenza A and influenza B viruses. The ENIA demonstrated sensitivities of 90.7% (147/162) for influenza A viruses and 81.80% (9/11) for influenza B viruses compared to those for an in-house reverse transcription (RT)-PCR assay in testing of influenza-positive clinical samples.
Url:
DOI: 10.1128/JCM.02635-14
PubMed: 25297327
PubMed Central: 4313323
Affiliations:
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type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Vemula, Sai Vikram" sort="Vemula, Sai Vikram" uniqKey="Vemula S" first="Sai Vikram" last="Vemula">Sai Vikram Vemula</name><affiliation wicri:level="2"><nlm:aff id="aff1">Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Zhao, Jiangqin" sort="Zhao, Jiangqin" uniqKey="Zhao J" first="Jiangqin" last="Zhao">Jiangqin Zhao</name><affiliation wicri:level="2"><nlm:aff id="aff1">Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, 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H" first="Haleyurgirisetty" last="Mohan">Haleyurgirisetty Mohan</name><affiliation wicri:level="2"><nlm:aff id="aff1">Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Liu, Jikun" sort="Liu, Jikun" uniqKey="Liu J" first="Jikun" last="Liu">Jikun Liu</name><affiliation wicri:level="2"><nlm:aff id="aff1">Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="El Mubarak, Haja Sittana" sort="El Mubarak, Haja Sittana" uniqKey="El Mubarak H" first="Haja Sittana" last="El Mubarak">Haja Sittana El Mubarak</name><affiliation wicri:level="2"><nlm:aff id="aff2">Center for Devices and Radiological Health, Food and Drug Administration, Silver Spring, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Devices and Radiological Health, Food and Drug Administration, Silver Spring, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Landry, Marie L" sort="Landry, Marie L" uniqKey="Landry M" first="Marie L." last="Landry">Marie L. Landry</name><affiliation wicri:level="2"><nlm:aff id="aff3">Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut</wicri:regionArea><placeName><region type="state">Connecticut</region></placeName></affiliation></author><author><name sortKey="Hewlett, Indira" sort="Hewlett, Indira" uniqKey="Hewlett I" first="Indira" last="Hewlett">Indira Hewlett</name><affiliation wicri:level="2"><nlm:aff id="aff1">Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author></analytic><series><title level="j">Journal of Clinical Microbiology</title><idno type="ISSN">0095-1137</idno><idno type="eISSN">1098-660X</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>We report the development of a novel europium nanoparticle-based immunoassay (ENIA) for rapid detection of influenza A and influenza B viruses. The ENIA demonstrated sensitivities of 90.7% (147/162) for influenza A viruses and 81.80% (9/11) for influenza B viruses compared to those for an in-house reverse transcription (RT)-PCR assay in testing of influenza-positive clinical samples.</p></div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Connecticut</li><li>Maryland</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Zhang, Panhe" sort="Zhang, Panhe" uniqKey="Zhang P" first="Panhe" last="Zhang">Panhe Zhang</name></region><name sortKey="Du, Bingchen" sort="Du, Bingchen" uniqKey="Du B" first="Bingchen" last="Du">Bingchen Du</name><name sortKey="El Mubarak, Haja Sittana" sort="El Mubarak, Haja Sittana" uniqKey="El Mubarak H" first="Haja Sittana" last="El Mubarak">Haja Sittana El Mubarak</name><name sortKey="Hewlett, Indira" sort="Hewlett, Indira" uniqKey="Hewlett I" first="Indira" last="Hewlett">Indira Hewlett</name><name sortKey="Landry, Marie L" sort="Landry, Marie L" uniqKey="Landry M" first="Marie L." last="Landry">Marie L. Landry</name><name sortKey="Liu, Jikun" sort="Liu, Jikun" uniqKey="Liu J" first="Jikun" last="Liu">Jikun Liu</name><name sortKey="Mohan, Haleyurgirisetty" sort="Mohan, Haleyurgirisetty" uniqKey="Mohan H" first="Haleyurgirisetty" last="Mohan">Haleyurgirisetty Mohan</name><name sortKey="Vemula, Sai Vikram" sort="Vemula, Sai Vikram" uniqKey="Vemula S" first="Sai Vikram" last="Vemula">Sai Vikram Vemula</name><name sortKey="Zhao, Jiangqin" sort="Zhao, Jiangqin" uniqKey="Zhao J" first="Jiangqin" last="Zhao">Jiangqin Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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